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Digestion, nutrition & metabolism

Complete genome sequence of the Streptococcus gallolyticus subsp. gallolyticus Strain DSM 16831

Grimm, I, Dumke, J, Vollmer, T, Hinse, D, Rückert, C, Kalinowski, J, Knabbe, C & Dreier, J 2017, Genome Announcements, vol. 5, no. 16,  e00108-17.

The complete genome sequence of Streptococcus gallolyticus subsp. gallolyticus strain DSM 16831, found in koala faeces and noted for its low virulence compared to other isolates, has been determined and reported here.

  Despite being a commensal bacterium within the human and animal gastrointestinal tract, S. gallolyticus subsp. gallolyticus can cause diseases such as septicaemia and meningitis. Strain DSM 16831 is the only one of 23 tested strains which is unable to establish a primary infection, as it is highly susceptible to host antimicrobial responses. In this study, to determine the contributory factors to its low virulence, S. gallolyticus subsp. gallolyticus strain DSM 16831 was isolated from the faeces of koalas and its genomic DNA sequenced. The completed genome was found to have a length of 2,492,900 base-pairs (bp) and a G+C content of 37.7%, with 2396 codons, 12 transfer RNAs (tRNAs) and 18 ribosomal RNAs (rRNAs). A total of 13 transposases were detected within the genome, with three regions identified to consist of phage-associated genes. One of the regions was noted to exhibit high similarity to the streptococcal phage P9. Further sequence analysis identified 11 genes that could be involved in antibiotic resistance of the bacterium. Additionally, genes associated with virulence, pil1 and pil3, were not found in the strain.

  Considering the low virulence of strain DSM 16831, it is relatively safe to work with in experimental procedures, as other strains of S. gallolyticus subsp. gallolyticus can cause disease in humans. Additionally, strain DSM 16831 can still induce similar antimicrobial responses to more pathogenic strains. Thus, the completed genome of strain DSM 16831 which would allow for comparative analysis into the genetics of virulence of pathogenic S. gallolyticus subsp. gallolyticus.

 

Summarised by Daniel Chew

 

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