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Comparison of Chlamydia psittaci Isolates by Restriction Endonuclease and DNA Probe Analyses


PETER TIMMS,1* FREDRICK W. EAVES,1 ADEEB A. GIRJES,2 AND MARTIN F. LAVIN2


1Animal Research Institute, Queensland Department of Primary Industries, Yeerongpilly, Queensland 4105,1 and
2Department of Biochemistry, University of Queensland, St. Lucia, Queensland 4067,2 Australia

 

ABSTRACT
DNAs from eight Chlamydia psittaci isolates (koala conjunctivitis, avian psittacosis, avian ornithosis, ovine
abortion, ovine polyarthritis, sporadic bovine encephalomyelitis, and feline conjunctivitis) and one Chlamydia
trachomatis isolate (lymphogranuloma venereum) were compared by restriction endonuclease and DNA probe
analyses. Digestion with HindIII yielded a series of discrete fragments which allowed the differentiation of most
isolates. A gene probe, pFEN207, which encodes the chlamydia-specific component of the Lipopolysaccharide
group antigen was used in Southern hybridizations. The probe was chlamydia-specific and hybridized to a
single BamHI fragment and multiple HindIII fragments in each isolate. The variation in size of the hybridizing
fragments allowed easy differentiation of the isolates and may eventually lead to a meaningful subgrouping of
the diverse group of disease agents presently included in the species C. psittaci.