Evaluation of an enzyme immunoassay test for the diagnosis of Chlamydia psittaci infection in free-ranging koalas (Phascolarctos Cinereus) in Southeastern Queensland, Australia
Benjamin J. Weigler,1,4 F. Christian Baldock,2 Adeeb A. Girjes,3 Frank N. Carrick,5 and Martin F. Lavin3
1 Department of Animal Sciences and Production, University of Queensland, St. Lucia, Queensland 4067, Australia
2 Animal Research Institute, Queensland Department of Primary Industries, 665 Fairfield Road, Yeerongpilly, Queensland 4105, Australia
3Department of Biochemistry, University of Queensland, St. Lucia, Queensland 4067, Australia
4Present address: Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
5Present address: Department of Zoology, University of Queensland, St. Lucia, Queensland4067, Australia
The IDEIA Chlamydia Test, a commercially available antigen-capture enzyme-linked immunosorbent assay (ELISA) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern Queensland determined to be infected with Chlamydia psittaci. Compared to isolation of the organism in tissue culture, the sensitivity of the IDEIA test ranged from 3 to 11%, and the specificity from 90 to 97%. The results indicated that the IDEIA test is unsuitable for use as a diagnostic screening test for C. psittaci in free-ranging koalas.