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Genetic Variation of Mitochondrial Cytochrome b Genes among the Subspecies of Koala, Phascolarctos cinereus


Kazutoshi TAKAMI1 Mitsutoshi YOSHIDA2, Yoshihiro YAMAMOTO3, Masashi HARADA4 and Jun-ichi FURUYAMA3


1Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529,

2Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065,

3Department of Genetics, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8131, and

4Laboratory Animal Center, Osaka City University Medical School, Abeno, Osaka 545¬0051, Japan


ABSTRACT

A conserved DNA region among the subspecies of koala, of mitochondrial cytochrome b gene, was employed to analyze the genetic variation among 3 available subspecies of koala. This conserved sequence, 307 by DNA, was sequenced using polymerase chain reaction and direct DNA sequencing technique. Substitutions in the nucleotide sequences were observed, with which koalas can be divided into 3 DNA haplotypes subspecies, but the molecular data provided inconsistency with current classification of the 3 subspecies of koala.