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Lonepinella koalarum gen. nov., sp. nov., a New Tannin-Protein Complex Degrading Bacterium

R. OSAWA1.5 F. RAINEY2, T. FUJISAWA3, E. LANG2, H.J. BUSSE4 T. P. WALSH5 and E. STACKEBRANDT2

1 Veterinary Service and Research, Lone Pine Koala Sanctuary, Fig Tree Pocket, Brisbane, 4069, Australia

Deutsche Sammlung von Mikroorganism und Zellkulturen GmbH, Mascheroder Weg 1 b, 0-38124 Braunschweig, Germany

3Kanagawa Prefectural Public Health Laboratory, 52-2, Nakao-cho, Asahi-ku, Yokohama, 241 Japan

4 Institute fur Microbiologie & Genetik, Universitat Wien, Dr. Bohr-Gasse 9, A-1030 Wien, Austria

Centre for Molecular Biotechnology, School of Life Science, Queensland University of Technology, Gardens Point Campus, Brisbane Queensland 4001, Australia

ABSTRACT

The phylogeny of bacteria that degrade a tannin-protein complex (T-PC) isolatcd from the fecal biota of koala was investigated. All T-PC degrading bacteria were facultatively anaerobic Gram-negative rods, producing tannase, but negative for catalase and oxidase activities. The 16S rONA sequence analyses of eight T-PC degrading strains representing biovars a, b, c, and d, revealed that the strains formed a phylogenetic cluster within the family Pasteurellaceae. Furthermore seven strains of biovar a, b, and c formed a 16S rONA sub-cluster with similarity values in the range 97.6 to 99.8%_ As the strains also exhibit a combination of chemotaxonomic and physiological properties not found in other members of the family Pasteurellaceae, it was concluded that the T-PC degrading isolates of biovar a, b, and c represent a new genus and species, for which the name Lonepinella koalarum gen. nov., sp. nov. is proposed.