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Recent developments in the laboratory diagnosis of chlamydial infections


Konrad Sachse a,*, Evangelia Vretou b, Morag Livingstone c, Nicole Borel d, Andreas Pospischil d, David Longbottom c


a Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Naumburger Str. 96a, 07743 Jena, Germany
b Hellenic Pasteur Institute, 127 Vassilittis Sofias, 11521 Athens, Greece
c Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK
d Institute of Veterinary Pathology, University of Zurich, Winterthurer Str. 268, 8057 Zurich, Switzerland

 

ABSTRACT
There are two main approaches to diagnosing infections by 
Chlamydia and Chlamydophila spp. in mammals and birds. The first involves the direct detection of the agent in tissue or swab samples, while the second involves the serological screening of blood samples for the presence of anti-chlamydial antibodies. Ultimately, the test that is used is dependent on the types of samples that are submitted to the diagnostic laboratory for analysis. The present paper gives an overview on methodologies and technologies used currently in diagnosis of chlamydial infections with emphasis on recently developed tests. The performance characteristics of individual methods, such as the detection of antigen in smears and in pathological samples, the isolation of the pathogen, various antibody detection tests and DNA-based methods utilising conventional and real-time PCR, as well as DNA microarray technology are assessed, and specific advantages and drawbacks are discussed. Further, a combination of a specific real-time PCR assay and a microarray test for chlamydiae is proposed as an alternative reference standard to isolation by cell culture.

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  • 2013
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