The roles of histology and immunohistology in the investigation of marsupial disease and normal lymphoid tissue
P.J. Canfield*, S. Hemsley
Department of Veterinary Anatomy and Pathology, University of Sydney, NSW 2006, Australia
This review acquaints scientists with current information related to the application of histology and immunohistology to the studies of normal lymphoid tissues and specific diseases in marsupials. Histological examination of tissue is a necessary prerequisite for immunohistological examination because it establishes tissue structure and detects specific areas of disease which allow the selection of smaller areas for immunohistological examination. Information is provided on the basic techniques of histology. Immunohistology (immunohistochemistry) refers to the identification of antigenic determinants of specific substances (proteins) by the application of antibodies to histological sections. Information is provided on a technique for enzyme based, avidin– biotin enhanced immunohistology, and on antibodies that can be used to mark disease agents or marsupial tissues and cells. A summary is provided of the available information on studies of the histology and immunohistology of normal marsupial lymphoid tissue (thymus, spleen, lymph nodes and mucosa associated lymphoid tissue), and of selected marsupial diseases such as chlamydiosis and lymphosarcoma in koalas.